Journal: PeerJ

Article Title: Characterization of the Polish Primitive Horse (Konik) maternal lines using mitochondrial D-loop sequence variation

doi: 10.7717/peerj.3714

Figure Lengend Snippet: The studied PPH maternal lines and the basic indices describing analyzed mtDNA D-loop sequence variation.

Article Snippet: Amplification of 510 bp fragment of the equine mitochondrial DNA sequence (nucleotide positions: 15343–15852, according to GenBank X79547 reference sequence), containing the HVR1 (hypervariable region 1) was carried out in 10 μl total volume containing: 1U of DreamTaq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA), 0.5 mM of each primer, 1 × PCR buffer, 0.2 mM of dNTPs and 50 ng of template DNA.

Techniques: Sequencing

Journal: PeerJ

Article Title: Characterization of the Polish Primitive Horse (Konik) maternal lines using mitochondrial D-loop sequence variation

doi: 10.7717/peerj.3714

Figure Lengend Snippet: The haplogroups were named according to . The sizes of the circles are proportional to the number of individuals carrying given haplotype. Black asterisks, PPH haplotypes tested in the present study (H_2—H_20); red asterisk, haplotype of the only PPH sequence derived from the GenBank database ( HQ439473 ); green asterisk, reference equine mtDNA sequence ( X79547 ).

Article Snippet: Amplification of 510 bp fragment of the equine mitochondrial DNA sequence (nucleotide positions: 15343–15852, according to GenBank X79547 reference sequence), containing the HVR1 (hypervariable region 1) was carried out in 10 μl total volume containing: 1U of DreamTaq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA), 0.5 mM of each primer, 1 × PCR buffer, 0.2 mM of dNTPs and 50 ng of template DNA.

Techniques: Sequencing, Derivative Assay